Apoptosis is a programmed cell death mechanism that is induced by a variety of factors that activate cysteine ​​proteases known as caspases, leading the cell to its final death.
The use of antibodies for apoptosis directed against specific proteins of the apoptotic pathway, is an essential tool for studying the role of apoptosis in different experimental models.
In this post we tell you how to identify the best antibodies for apoptosis, as well as other related proteins and ELISA kits. But before we learn a little more about this cell death mechanism.
REGULATION OF APOPTOSIS
The correct regulation of apoptosis is fundamental for the proper development of the organs and their homeostasis, as well as a fundamental part of immunity.
It is a normal and essential biological process, for example, for the proper development of organs during embryogenesis or the elimination of abnormal cells such as tumor cells or those damaged by pathogens or toxic stimuli.
However, premature or unscheduled apoptosis can lead to loss of functional cells, which could lead to autoimmune, neurological, or cardiovascular disorders.
DIFFERENCES BETWEEN APOPTOSIS, NECROSIS AND AUTOPHAGY
The apoptosis or programmed cell death, is by necrosis and autophagy one of the mechanisms that cause cell death. The main difference between these three mechanisms are the morphological changes at the cellular level that take place next to each one of them, as well as the proteins involved in their activation and execution.
In the case of apoptosis, the most characteristic changes in cell morphology are usually cell contraction, chromatin condensation, nuclear fragmentation, and cell rupture with the consequent formation of apoptotic bodies.
The main proteins involved in activating apoptotic pathways are caspases and the p53 protein.
APOPTOSIS ANTIBODIES
As in so many other areas of study, the use of correctly validated apoptosis antibodies is critical to achieving robust and reproducible results.
Once the protein or proteins of interest for the investigation have been identified, it is convenient to identify and select quality reagents, which have undergone exhaustive validation procedures that guarantee success in a given experimental application.
As an example we bring you one of the proteins that is most frequently analyzed using antibodies for apoptosis: Caspase 9 (CASP9) .
After a search for antibodies to apoptosis and other validated bioreactants (in this case we have focused on reactivity in humans), we obtain the following selection:
| Reference | Name | Applications for which it is validated | |
| Proteins | ABK1-P2614 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. |
| ABK1-P2615 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| ABK1-P2616 | Recombinant Caspase 9 (CASP9) | Positive Control; Immunogen; SDS-PAGE; WB. | |
| Antibodies | ABK1-A1599 | Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. |
| ABK1-A7669 | Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. | |
| ABK1-A1393 | Polyclonal Antibody to Caspase 9 (CASP9) | WB; IHC; ICC; IP. | |
| ELISA kits | ABK1-E184 | ELISA Kit for Caspase 9 (CASP9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
These antibodies, proteins and ELISA kits are validated for the techniques indicated in each case, ensuring their good performance based on the established procedure.
In case you are interested in other antibodies to apoptosis or any other area of research , do not hesitate to contact us and our team will advise on the best validated antibodies and guaranteed for each case.